Xetrabiosolution

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      Gene expression / Genotyping

      We offer the Gold standard Taq man Chemistry and the most commonly used SYBR green chemistry

      Processing Procedures

      1. Isolation and purification of RNA 
      2. Quantification of RNA using  Nano-drop
      3. RNA to cDNA conversion
      4. Gene specific primers/ probe synthesis
      5. Primer efficiency calculation
      6. Housekeeping gene synthesis (depending upon the type of sample)
      7. Relative quantification  or absolute quantification.

       

      Samples Accepted

      1. Pure Culture / Nucleic acids
      2. Gene Information in FASTA Format
       
      Services include
      1. SNP genotyping
      2. Microsatellite Genotyping
      3. PCR Markers

      We provide Best Solution for the gene screening for bulk samples.

      RAPD, SSR and ISSR based markers for studying polymorphism between samples

      We Accept

      Crude samples / Genomic DNA 

      primers or primer sequences

      Genotyping using PCR amplified DNA fragments with Fluorescent tags. After checking the product on the Gel , the sample is further diluted to avoid more signals. To the Diluted product the Hi-Di Formamide along with suitable size standard and run on sequencer after proper optimisation.

      Samples accepted 

      • Purified / unpurified tagged PCR products 
      • Genomic DNA 

      SNPs (single nucleotide polymorphisms) or point mutations are the most common types of genetic variation determining to a major part the phenotype diversity between individuals.

      • We use two different approaches one by using SNP Shot Kit based or by using General sanger Sequencing method.

       

      Samples accepted 

      • Genomic DNA