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      Oligo Design & more

      We offer primer design services and customized conceptions for large-scale oligos.

      The primer design is essential for the success of your application. We offer free primer design and calculation tools as well as individual design project services.

      • Oligo Property Scan tool
        Analysis and calculation of different physical properties of your DNA and RNA oligo sequence
      • Primer design tools
        Online design of primers and probes for applications such as PCR, DNA sequencing, qPCR, and FRET
      • Oligo design projects
        For projects requiring several hundreds of oligos for applications such as PCR, RT-PCR, qPCR, or microarray experiments

      Feel free to contact us for your large-scale oligo project, which requires large amounts of DNA or RNA oligos.

      We also offer double-stranded DNA of any length and the highest quality with our gene synthesis service.

       

      Medium- to large-scale DNA and RNA oligonucleotides

      Requirements for oligos in mg-to-gram quantity ranges are fulfilled with our large-scale oligo service.

      Large-scale oligos are custom-ordered from multi-milligram to gram quantities.

      • A fully customized service
      • Consistent high quality without batch variations
      • There are different purification options available
      • All oligo products are analysed by MALDI-TOF MS and CGE

      Please request a quotation for your large-scale oligo (please drop a mail to us or contact our local RMs):

      • Phosphorthioate, phosphodiester backbone, or a combination of both
      • A wide variety of oligo modifications are available on request
      • Normalization and aliquoting on option

      Turnaround times depend on the sequence, purification, and quantity requested.

       

      The one-stop solution for quick and easy oligo analysis

      Our multifunctional oligo property analysis tool (MOPS) is an oligo calculator inclusive a dilution calculator, a TM calculator and much more!

      MOPS allows you to analyse and calculate any physical property of your DNA or RNA oligo sequence:

      • Physical property check incl. TM calculator
        Determines all physical properties such as GC content, melting temperature (Tm), molecular weight (MW), extinction coefficient (EC) and complement sequences
      • Secondary structures
        Predict the secondary structure pf DNA and RNA oligos using a mfold and RNAfold program. Mfold is an adaptation of the mfold package by Zuker and Jaeger which was modified to work with the Wisconsin Package.
      • BLASTn analysis
        Aligns your sequence against human, mouse and rat Refseq database entries and provides a comprehensive BLASTn report.
      • Dimer formation & PCR viability check
        Tests your oligo against itself or another oligo for dimer formation and performs a PCR viability check for a primer pair
      • OD calculator
        Calculates the optical density (OD), mass, amount and molecular weight (MW) of your oligo for specific experimental conditions. The appropriate oligo synthesis scale can also be decipered.
      • Dilution calculator
        Determines how much volume is needed to dilute a highly concentraged oligo stock solution to a specific lower concentration. This application also supports different units for concentration and volume.

      All these analyses can be done with a single free online tool that includes a direct link to the respective oligo order menu.

      The secret to successful primer and probe design

      Precise primer and probe design is essential for successful PCR reactions.

      The optimum design parameters are the key to specific amplification and are set as default in our online design tools:

      • PCR primer design
        Specific design of primers for standard PCR, RT-PCR (reverse transcriptase), and DNA sequencing.
      • qPCR primer and probe design
        Optimal primers and probes for single-plex real-time qPCR applications.
      • Bi-Plex qPCR Assay design
        Perform the design of primer and probe sets for two target regions for multiplex qPCR analyses.
      • LightCycler probe design
        Design of specific primers and probes for FRET assays.

      The results are scored according to the best-predicted performance criteria.

      Selected primers and probes can be transferred directly to your online shopping cart

      Your specific oligo design project in best hands

      Our bioinformatic team has comprehensive experience in performing oligo design projects and offers individual solutions for your requirements.

      Your design projects are handled confidential and quickly

      Our oligo design project services

      • include a highly specific comparison with our proprietary data bases.
      • are absolutely secure as all comparisons are performed in-house.
      • are supported by a powerful computing centre and IT team.

      For oligo design projects with several hundred oligonucleotides, manual design can often be time consuming, unmanageable and error ridden.

      We have the know-how and resources to support you by

      • PCR primer design for a defined number of genes up to complete transcriptomes.
      • qPCR primer and probe design for a defined number of genes.
      • PCR primer design for the amplification of a specific amount of genes or RNA sequences, e.g. splice variants.
      • Oligonucleotide design for microarray experiments.