786-035 Proteinase K, Lyophilized 250mg

Proteinase K (also protease K, endopeptidase K, peptidase K or Tritirachium  alkaline phosphatase) (EC 3.4.21.64) is a non-specifc, broad spectrum serine protease that is isolated from the saprophytic fungus Tritirachium album.

Proteinase K is routinely used for the purification of target material from contaminating proteins, for the isolation of RNA and DNA and to inactivate other protein enzymatic activities. Proteinase K exhibits high activity in the presence of SDS, urea and EDTA. This allows the use of EDTA in the purification of DNA or RNA to inhibit calcium dependent nucleases.

The dry, lyophilized powder is highly soluble (>50mg/ml); however, stock solutions are normally 20mg/ml. The proteinase K is completely RNase and DNase free and has a specific activity of >30 units/mg at 37°C. The recommended storage buffer is 20mM Tris-HCl (pH7.4), 1mM CaCl2, 50% glycerol. Proteinase K is stable for 1 year in this storage buffer, recommended storage is -20°C.

LongLife™ Proteinase K is an optimized, 5mg/ml solution that is free from all nuclease contamination. The specific activity is >30 units/mg at 37°C. The buffering conditions used ensure that the enzyme retains its full activity and offers convenience to the researchers. LongLife™ Proteinase K is stable for a year if stored correctly.

All of our LongLife™ enzyme preparations have a long shelf life and no weighing or buffer preparation is needed; simply take an aliquot and add to your sample. LongLife™ enzyme preparations contain cofactors necessary for optimal enzymatic activity. Supplied in suspension form and when stored properly have a one year shelf life.